Scientists Assess Kidney Damage with Stereo Investigator, Determine Ultrasound Prevents Organ Injury

Using the virtual tissue function of Stereo Investigator a series of photos of each section was captured using the 5X objective and automatically stitched together to form a montage of the entire section of kidney that clearly represents the degree of injury.  Kidneys are from mice treated either with vehicle (0.3 M sodium bicarbonate) or a single injection of folic acid (250 mg/kg, ip) in sodium bicarbonate, and mice were euthanized after 14 days. Injection of folic acid has been used as one model of chronic kidney disease. The degree of fibrosis in the kidney, revealed here by Masson’s trichrome stain (blue: collagen deposition), was determined using the area fraction fractionator probe in Stereo Investigator.

Using the Virtual Tissue module with Stereo Investigator, a series of photos of each kidney section was captured using the 5X objective and then automatically stitched together to form a montage of the entire section of kidney that clearly represents the degree of injury.  Kidneys are from mice treated either with vehicle (0.3 M sodium bicarbonate) or a single injection of folic acid (250 mg/kg, ip) in sodium bicarbonate. The mice were euthanized after 14 days. Injection of folic acid has been used as one model of chronic kidney disease. The degree of fibrosis in the kidney, revealed here by Masson’s trichrome stain (blue: collagen deposition), was determined using the area fraction fractionator probe in Stereo Investigator.

When it comes to health, kidneys are critical. From regulating blood composition to maintaining calcium levels, the pair of bean-shaped organs perform several essential tasks. Needless to say, interruption to kidney function can be disastrous.

Working with scientists in South Korea, researchers at the University of Virginia found a surprisingly simple treatment for renal ischemia-reperfusion injury (IRI) in mice, which is a model of acute kidney injury (AKI) in humans.

The researchers found that mice exposed to ultrasound prior to IRI “had preserved kidney structure and function accompanied by a reduction in tissue inflammation,” they report in their paper published this month in the Journal of the American Society of Nephrology.

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