New Software Released for Automated Neuron Reconstruction and Analysis

montage for email invitationHow the brain works and how the brain is affected by disease are mysteries in large part because neurons are so dynamic, numerous, and complex. Neurolucida 360, a revolutionary, new software product from MBF Bioscience, enables neuroscientists to uncover more information about neurons at a faster rate.

“Neurolucida 360 is a technological revolution” says Jack Glaser, President of MBF Bioscience. “It is the state-of-the-art tool for neuroscientists to analyze the shape and connectivity of neurons more quickly and accurately than has ever been possible, so that we can better understand the brain and the mechanisms behind diseases such as Alzheimer’s and Parkinson’s. With the unique ability to automatically detect and analyze dendrites, dendritic spines, axons, somas, and synapses, Neurolucida 360 is now the standardized platform for the global neuroscience research community to perform unprecedented investigations into the functioning of the brain.”

Using automated tools in Neurolucida 360, researchers generate high-resolution, digital 3D reconstructions of neurons imaged with numerous microscopy techniques, including light sheet, 2 photon, confocal and brightfield. Using the most advanced algorithms for neuron reconstruction, researchers instantly receive hundreds of analyses about the size, shape, and complexity of neurons. The reliable data from Neurolucida 360 is integral to learning how injury, disease, or chemicals change neuronal structure, discovering how neuronal structure affects function, finding out which brain regions neurons communicate with, and more.

The National Institute of Mental Health provides funding to support the development of Neurolucida 360. It is the latest development in the renowned legacy of neuron tracing tools that started with Neurolucida – the most widely cited tool for neuron reconstruction and analysis.

For more information on Neurolucida 360, please visit our website or watch this short video.

Stereology Workshop at Obafemi Awolowo University in Nigeria

CaptureStereology Symposium in Nigeria

Dr. Jose Maldonado, Head of MBF Bioscience Latin America and Africa, and Dr. Abraham A.A. Osinubi, Associate Professor, University of Lagos will be hosting a stereology workshop at Obafemi Awolowo University in Nigeria on Sept 16 – 18. The workshop will cover the basics of stereology and how to practically apply it to scientific research.

This is a unique opportunity for attendees to learn from two expert stereologists. Anyone interested in stereology is welcome to attend, including undergraduate and post-graduate students and faculty/researchers from biological and material science, geologists, pathologists, and statisticians.

To register for this workshop, send an email to stereologynigeria@gmail.com stating your full name, institution, email address, phone number and status (Student/Faculty).

More information can be found here in the workshop brochure.

Satellite Symposium at SfN: Enhancing the Reproducibility of Your Research Results with Stereology

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Recent headlines decry the alarming amount of irreproducible data in published research papers. MBF Bioscience is hosting a symposium addressing the topic on Sunday, October 18 at 6:30pm entitled “Quantitative Microscopy: Enhancing the Reproducibility of Your Research Results with Stereology.” This symposium, which is a satellite event at the annual Society for Neuroscience meeting, will address how researchers can use stereology to obtain accurate, reproducible data about their histological tissue specimens to drive meaningful discovery by the scientific community at large.

It will be an evening full of engaging speakers with significant expertise in various aspects of stereology, including: stereology theory, the application of stereology in neuroscience research, and why some funding agencies require stereology. Attendees will have a chance to interact with the speakers during the question and answer session following the presentations.

Stereology is the gold standard method for quantifying the number of cells, length of fibers, and the area and volume of structures or regions in tissue specimens. Attend this symposium to learn how it can help you obtain accurate, reproducible data for your research study.

Webinar Video: Automated 3D Neuron Reconstruction

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Confocal image of a mouse cerebellum optically cleared with CLARITY. Neurons were reconstructed with Neurolucida 360.

 

In this webinar, Dr. Dara Dickstein, Assistant Professor at Icahn School of Medicine at Mount Sinai, and Dr. Susan Tappan, Staff Scientist and Neurolucida 360 Product Manager discuss imaging protocols and techniques for automated 3D neuron reconstruction.

Watch the webinar on YouTube

The goal is to help you develop an imaging and quantification strategy for your own research to increase the throughput of your morphologic study.

Watch this webinar to learn:

+ Different methods for tissue preparation
+ Imaging protocols optimized for automated neuron reconstruction
+ How to use Neurolucida 360 – our new automated software for achieving accurate 3D reconstructions of somas, dendrites, spines, and axons

 

How Transplanted Stem Cells Behave in Injured Spinal Cord Tissue

A representative confocal image of spinal cord tissue fluorescently immunolabeled for SC121 in conjunction with GFAP – markers that allowed the researchers to track stem cell differentiation and migration by stereological quantification. (Image provided by study author Dr. Aileen J. Anderson)

A representative confocal image of spinal cord tissue fluorescently immunolabeled for SC121 (red) in conjunction with GFAP (green) – markers that allowed researchers to quantify stem cell differentiation and migration. (Image provided by study author Dr. Aileen J. Anderson)

Research has shown that transplanting human neural stem cells into damaged spinal cords restores locomotor function in a mouse model of spinal cord injury1. Researchers who worked on that study have published another paper examining how these neural stem cells behave in injured tissue as they aid in healing. Learning how stem cells behave in injured tissue will hopefully help researchers develop better treatments for spinal cord injuries.

In the study, researchers used Stereo Investigator to stereologically quantify the survival, migration, proliferation, and differentiation of human neural stem cells transplanted into injured and uninjured mice. Stem cells were analyzed in mouse brain tissue specimens 1, 7, 14, 28, and 98 days after transplantation. The research found that there were fewer stem cells in the injured animals compared to the uninjured animals at all time points, stem cells in injured mice localized near the center of the injury, a delay of stem cell proliferation in injured tissue led to an overall deficit of actively dividing cells, proliferation in injured mice occurred closer to the injection sites (the locations where the stem cells were injected into the mice), and the injured microenvironment increased differentiation to more mature oligodendrocytes.

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Researchers cited MBF systems in 7 papers during the week of 1/12/15

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Friess, S. H., Lapidus, J. B., & Brody, D. L. (2015). Decompressive craniectomy reduces white matter injury following controlled cortical impact in mice. Journal of Neurotrauma. doi: 10.1089/neu.2014.3564.

González-Cabrera, C., Garrido-Charad, F., Roth, A., & Marín, G. J. (2015). The isthmic nuclei providing parallel feedback connections to the avian tectum have different neurochemical identities: Expression of Glutamatergic and Cholinergic Markers in the chick (Gallus gallus). Journal of Comparative Neurology, n/a-n/a. doi: 10.1002/cne.23739. http://dx.doi.org/10.1002/cne.23739

Isgor, C., Pare, C., McDole, B., Coombs, P., & Guthrie, K. (2015). Expansion of the dentate mossy fiber–CA3 projection in the brain-derived neurotrophic factor-enriched mouse hippocampus. Neuroscience, 288(0), 10-23. doi: http://dx.doi.org/10.1016/j.neuroscience.2014.12.036.

Schaakxs, D., Kalbermatten, D. F., Pralong, E., Raffoul, W., Wiberg, M., & Kingham, P. J. (2014). Poly-3-hydroxybutyrate strips seeded with regenerative cells are effective promoters of peripheral nerve repair. Journal of Tissue Engineering and Regenerative Medicine, n/a-n/a. doi: 10.1002/term.1980.

Neurolucida:

Kecskes, S., Matesz, C., Gaál, B., & Birinyi, A. (2015). Neural circuits underlying tongue movements for the prey-catching behavior in frog: distribution of primary afferent terminals on motoneurons supplying the tongue. Brain Structure and Function, 1-21. doi: 10.1007/s00429-014-0988-1.

Medalla, M., & Luebke, J. I. (2015). Diversity of Glutamatergic Synaptic Strength in Lateral Prefrontal versus Primary Visual Cortices in the Rhesus Monkey. The Journal of Neuroscience, 35(1), 112-127.

Sun, C., Dayal, A., & Hill, D. L. (2015). Expanded Terminal Fields of Gustatory Nerves Accompany Embryonic BDNF Overexpression in Mouse Oral Epithelia. The Journal of Neuroscience, 35(1), 409-421.

Researchers cited MBF systems in 16 papers during the week of 10/12/14

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Acker, S. N., Mandell, E. W., Sims-Lucas, S., Gien, J., Abman, S. H., & Galambos, C. (2014). Histologic Identification of Prominent Intrapulmonary Anastomotic Vessels in Severe Congenital Diaphragmatic Hernia. The Journal of Pediatrics(0).

Dixon, K. J., Theus, M. H., Nelersa, C. M., Mier, J., Travieso, L. G., Yu, T.-S., . . . Liebl, D. J. (2014). Endogenous neural stem/progenitor cells stabilize the cortical microenvironment following traumatic brain injury. Journal of Neurotrauma.

Janelidze, S., Nordström, U., Kügler, S., & Brundin, P. (2014). Pre‐existing immunity to AAV2 limits transgene expression following intracerebral AAV2‐based gene delivery in a 6‐OHDA model of Parkinson’s disease. The Journal of Gene Medicine.

Le Belle, Janel E., Sperry, J., Ngo, A., Ghochani, Y., Laks, D. R., López-Aranda, M., . . . Kornblum, Harley I. (2014). Maternal Inflammation Contributes to Brain Overgrowth and Autism-Associated Behaviors through Altered Redox Signaling in Stem and Progenitor Cells. Stem Cell Reports(0).

Polinski, N. K., Gombash, S. E., Manfredsson, F. P., Lipton, J. W., Kemp, C. J., Cole-Strauss, A., . . . Sortwell, C. E. (2014). Recombinant adeno-associated virus 2/5-mediated gene transfer is reduced in the aged rat midbrain. Neurobiology of Aging. doi: 10.1016/j.neurobiolaging.2014.07.047

Continue reading “Researchers cited MBF systems in 16 papers during the week of 10/12/14” »

Researchers cited MBF systems in 12 papers during the week of 8/10/2014

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Chou, J.-S., Chen, C.-Y., Chen, Y.-L., Weng, Y.-H., Yeh, T.-H., Lu, C.-S., . . . Wang, H.-L. (2014). (G2019S) LRRK2 causes early-phase dysfunction of SNpc dopaminergic neurons and impairment of corticostriatal long-term depression in the PD transgenic mouse. Neurobiology of Disease, 68(0), 190-199.

Claes, J. M., Partridge, J. C., Hart, N. S., Garza-Gisholt, E., Ho, H.-C., Mallefet, J., & Collin, S. P. (2014). Photon Hunting in the Twilight Zone: Visual Features of Mesopelagic Bioluminescent Sharks. Plos one, 9(8), e104213. doi: 10.1371/journal.pone.0104213.

Kermer, P., Köhn, A., Schnieder, M., Lingor, P., Bähr, M., Liman, J., & Dohm, C. (2014). BAG1 is Neuroprotective in In Vivo and In Vitro Models of Parkinson’s Disease. Journal of Molecular Neuroscience, 1-9. doi: 10.1007/s12031-014-0396-2.

Loane, D., Stoica, B., Tchantchou, F., Kumar, A., Barrett, J., Akintola, T., . . . Faden, A. (2014). Novel mGluR5 Positive Allosteric Modulator Improves Functional Recovery, Attenuates Neurodegeneration, and Alters Microglial Polarization after Experimental Traumatic Brain Injury. Neurotherapeutics, 1-13.

Magen, I., Ostritsky, R., Richter, F., Zhu, C., Fleming, S. M., Lemesre, V., . . . Chesselet, M.-F. (2014). Intranasal NAP (davunetide) decreases tau hyperphosphorylation and moderately improves behavioral deficits in mice overexpressing α-synuclein. Pharmacology Research & Perspectives, 2(5), n/a-n/a.

Semple, B. D., Noble-Haeusslein, L. J., Jun Kwon, Y., Sam, P. N., Gibson, A. M., Grissom, S., . . . Schenk, A. K. (2014). Sociosexual and Communication Deficits after Traumatic Injury to the Developing Murine Brain. Plos one, 9(8), e103386.

Continue reading “Researchers cited MBF systems in 12 papers during the week of 8/10/2014” »

Researchers cited MBF systems in 30 papers during the week of 6/8/2014

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Barkan, S., Yom-Tov, Y., & Barnea, A. (2014). A possible relation between new neuronal recruitment and migratory behavior in Acrocephalus warblers. Developmental Neurobiology, n/a-n/a. doi: 10.1002/dneu.22198.

Bethea, C. L., Coleman, K., Phu, K., Reddy, A. P., & Phu, A. (2014). Relationships between androgens, serotonin gene expression and innervation in male macaques. Neuroscience(0).

Coimbra, J. P., Collin, S. P., & Hart, N. S. (2014). Topographic specializations in the retinal ganglion cell layer correlate with lateralized visual behavior, ecology and evolution in cockatoos. Journal of Comparative Neurology, n/a-n/a. doi: 10.1002/cne.23637.

Dellarole, A., Morton, P., Brambilla, R., Walters, W., Summers, S., Bernardes, D., . . . Bethea, J. R. (2014). Neuropathic pain-induced depressive-like behavior and hippocampal neurogenesis and plasticity are dependent on TNFR1 signaling. Brain, Behavior, and Immunity(0).

Höfling, C., Indrischek, H., Höpcke, T., Waniek, A., Cynis, H., Koch, B., . . . Hartlage-Rübsamen, M. (2014). Mouse strain and brain region-specific expression of the glutaminyl cyclases QC and isoQC. International Journal of Developmental Neuroscience(0).

Koh, M. T., Spiegel, A. M., & Gallagher, M. (2014). Age-associated changes in hippocampal-dependent cognition in Diversity Outbred mice. Hippocampus, n/a-n/a. doi: 10.1002/hipo.22311.

Continue reading “Researchers cited MBF systems in 30 papers during the week of 6/8/2014” »

Researchers cited MBF systems in papers 13 during the week of 5/12/2014

Stereo Investigator:

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Akers, K. G., Martinez-Canabal, A., Restivo, L., Yiu, A. P., De Cristofaro, A., Hsiang, H.-L. L., . . . Shoji, H. (2014). Hippocampal Neurogenesis Regulates Forgetting During Adulthood and Infancy. Science, 344(6184), 598-602.

Burger, D. K., Gulbrandsen, T., Saucier, D. M., & Iwaniuk, A. N. (2014). The effects of season and sex on dentate gyrus size and neurogenesis in a wild rodent, Richardson’s ground squirrel (Urocitellus richardsonii). Neuroscience(0).

Coimbra, J. P., Collin, S. P., & Hart, N. S. (2014). Topographic specializations in the retinal ganglion cell layer of Australian passerines. Journal of Comparative Neurology, n/a-n/a. doi: 10.1002/cne.23624.

Fernando, C., Kele, J., Bye, C., Niclis, J. C., Alsanie, W., Blakely, B. D., . . . Parish, C. (2014). Diverse roles for Wnt7a in ventral midbrain neurogenesis and dopaminergic axon morphogenesis. Stem Cells and Development(ja).

Gombash, S., Manfredsson, F., Mandel, R., Collier, T., Fischer, D., Kemp, C., . . . Sortwell, C. (2014). Neuroprotective potential of pleiotrophin overexpression in the striatonigral pathway compared with overexpression in both the striatonigral and nigrostriatal pathways. Gene Therapy.

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