Take a Look at the Latest Research Using MBF Systems (week of 1/21/13)



Our systems were mentioned in 11 published research papers last week alone.  Take a closer look at some of the research…



Stereo Investigator:


  • Bories, C., Husson, Z., Guitton, M. J., & De Koninck, Y. (2013). Differential Balance of Prefrontal Synaptic Activity in Successful versus Unsuccessful Cognitive Aging. The Journal of Neuroscience33(4), 1344-1356. doi: 10.1523/​JNEUROSCI.3258-12.2013
  • Kim, J. Y., Ash, R. T., Ceballos‐Diaz, C., Levites, Y., Golde, T. E., Smirnakis, S. M., & Jankowsky, J. L. (2013). Viral transduction of the neonatal brain delivers controllable genetic mosaicism for visualising and manipulating neuronal circuits in vivo. European Journal of Neuroscience. doi: 10.1111/ejn.12126
  • Morel, A., Gallay, M. N., Baechler, A., Wyss, M., & Gallay, D. S. (2013). The human insula: architectonic organization and postmortem mri registration. Neuroscience. doi:10.1016/j.neuroscience.2012.12.076
  • Poller, W. C., Madai, V. I., Bernard, R., Laube, G., & Veh, R. W. (2013). A glutamatergic projection from the lateral hypothalamus targets VTA-projecting neurons in the lateral habenula of the rat. Brain Research. doi: 10.1016/j.brainres.2013.01.029
  • Song, C. H., Bernhard, D., Bolarinwa, C., Hess, E. J., Smith, Y., & Jinnah, H. A. (2013). Subtle microstructural changes of the striatum in a DYT1 knock-in mouse model of dystonia. Neurobiology of Disease. doi: 10.1016/j.nbd.2013.01.008


Take a Look at the Latest Research Using MBF Systems (week of 1/14/13)



Our systems were mentioned in 12 published research papers last week alone.  Take a closer look at some of the research…




Stereo Investigator:

  • Bäck, S., Peränen, J., Galli, E., Pulkkila, P., Lonka-Nevalaita, L., Tamminen, T., Voutilainen, M. H., Raasmaja, A., Saarma, M., Männistö, P. T. and Tuominen, R. K. (2013). Gene therapy with AAV2-CDNF provides functional benefits in a rat model of Parkinson’s disease. Brain and Behavior. doi: 10.1002/brb3.117
  • Cimino, P. J., Yang, Y., Li, X., Hemingway, J. F., Cherne, M. K., Khademi, S. B., … & Keene, C. D. (2013). Ablation of the microglial protein DOCK2 reduces amyloid burden in a mouse model of Alzheimer’s disease. Experimental and Molecular Pathology. doi: 10.1016/j.yexmp.2013.01.002
  • Johansson, J., Mannerås-Holm, L., Shao, R., Olsson, A., Lönn, M., Billig, H., & Stener-Victorin, E. (2013). Electrical vs Manual Acupuncture Stimulation in a Rat Model of Polycystic Ovary Syndrome: Different Effects on Muscle and Fat Tissue Insulin Signaling. PLOS ONE8(1), e54357. doi:10.1371/journal.pone.0054357
  • Kalm, M., Karlsson, N., Nilsson, M. K., & Blomgren, K. (2013). Marie Kalm, Niklas Karlsson, Marie KL Nilsson, Klas Blomgren. Experimental Neurology. doi: 10.1016/j.expneurol.2013.01.006
  • Luk, K. C., Rymar, V. V., Munckhof, P., Nicolau, S., Steriade, C., Bifsha, P., … & Sadikot, A. F. (2013). The transcription factor Pitx3 is expressed selectively in midbrain dopaminergic neurons susceptible to neurodegenerative stress. Journal of Neurochemistry. doi: 10.1111/jnc.12160
  • Pranski, E. L., Dalal, N. V., Sanford, C. V., Herskowitz, J. H., Gearing, M., Lazo, C., … & Betarbet, R. S. (2013). RING finger protein 11 (RNF11) modulates susceptibility to 6-OHDA-induced nigral degeneration and behavioral deficits through NF-κB signaling in dopaminergic cells. Neurobiology of Disease. doi: 10.1016/j.nbd.2012.12.018
  • Su, H., Wang, L., Huang, W., Qin, D., Cai, J., Yao, X., … & Pei, D. (2013). Immediate expression of Cdh2 is essential for efficient neural differentiation of mouse induced pluripotent stem cells. Stem Cell Research. doi: 10.1016/j.scr.2013.01.003

Take a Look at the Latest Research Using MBF Systems (week of 1/7/13)



Our systems were mentioned in 8 published research papers last week.  Take a closer look at some of the research…



  • Browning, K. N., Babic, T., Holmes, G. M., Swartz, E. M., & Travagli, R. A. (2013). A critical re-evaluation of the specificity of action of perivagal capsaicin. The Journal of Physiology. doi:10.1113/jphysiol.2012.246827
  • Carlo, C. N., & Stevens, C. F. (2013). Structural uniformity of neocortex, revisited. Proceedings of the National Academy of Sciences. doi:10.1073/pnas.1221398110
  • Carrasco, A., Brown, T. A., Kok, M. A., Chabot, N., Kral, A., & Lomber, S. G. (2013). Influence of Core Auditory Cortical Areas on Acoustically Evoked Activity in Contralateral Primary Auditory Cortex. The Journal of Neuroscience,33(2), 776-789. doi:10.1523/​JNEUROSCI.1784-12.2013
  • Levine, N. D., Rademacher, D. J., Collier, T. J., O’Malley, J. A., Kells, A. P., San Sebastian, W., … & Steece-Collier, K. (2013). Advances in thin tissue Golgi-Cox impregnation: Fast, reliable methods for multi-assay analyses in rodent and non-human primate brain. Journal of Neuroscience Methods. doi: 10.1016/j.jneumeth.2012.12.001
  • Reid, G. A., Chilukuri, N., & Darvesh, S. (2013). Butyrylcholinesterase and the Cholinergic System. Neuroscience. doi: 10.1016/j.neuroscience.2012.12.054

Stereo Investigator:

  • Alexander, M. L., Hill, C. A., Rosenkrantz, T. S., & Fitch, R. H. (2013). Evaluation of the Therapeutic Benefit of Delayed Administration of Erythropoietin following Early Hypoxic-Ischemic Injury in Rodents. Developmental Neuroscience. doi: 10.1159/000345645
  • David, B. T., Ratnayake, A., Amarante, M. A., Reddy, N. P., Dong, W., Sampath, S., … & Elkabes, S. (2013). A toll-like receptor 9 antagonist reduces pain hypersensitivity and the inflammatory response in spinal cord injury. Neurobiology of Disease. doi: 10.1016/j.nbd.2012.12.012
  • Piao, C. S., Stoica, B. A., Wu, J., Sabirzhanov, B., Zhao, Z., Cabatbat, R., … & Faden, A. I. (2013). Late exercise reduces neuroinflammation and cognitive dysfunction after traumatic brain injury. Neurobiology of Disease. doi: 10.1016/j.nbd.2012.12.017

MBF Labs Contributes to Recent PLOS Article on New Mouse Model for Huntington’s Disease


Stereologic volume estimation of the striatum (caudate putamen and nucleus accumbens) confirms changes observed using MRI volumetry in zQ175 knock-in mice.

Stereologic volume estimation of the striatum (caudate putamen and nucleus accumbens) confirms changes observed using MRI volumetry in zQ175 knock-in mice. Stereological analyses showed significant decreases in neuron numbers in homozygous zQ175 knock-in mice aged 4.5 and 10 months (13% and 15% respectively) as compared to age-matched wild-type mice

There is very little known about Huntington’s disease (HD), a fatal neurodegenerative disease leading to total physical and mental decline that affects 30,000 Americans today.

Researchers have been developing transgenic mouse models to mimic human HD. A new model, the zQ175 knock-in, developed by Menalled et al. (2012) appears to more closely mimic human HD progression in the zQ175 KI than previous mouse models.

A recently published paper (co-authored by MBF’s President, Jack Glaser, and Staff Scientist Dr. Susan Hendricks) confirms the behavioral phenotypes reported by Menalled, and extends the characterization to include brain volumetry, striatal metabolite concentration, and early neurophysiological changes. For this study, MBF Labs used Stereo Investigator to quantify neural loss and regional volumetric changes. These changes validate the similarities between HD progression in humans and HD-like progression in zQ175 KI mice.

With this new mouse model, researchers might be able to considerably improve their understanding of the disease to find ways to temper HD progression.

Read the full paper “Characterization of Neurophysiological and Behavioral Changes, MRI Brain Volumetry and 1H MRS in zQ175 Knock-In Mouse Model of Huntington’s Disease” on PLOS.



Heikkinen T, Lehtimäki K, Vartiainen N, Puoliväli J, Hendricks SJ, et al. (2012). Characterization of Neurophysiological and Behavioral Changes, MRI Brain Volumetry and 1H MRS in zQ175 Knock-In Mouse Model of Huntington’s Disease. PLoS ONE 7(12): e50717. doi:10.1371/journal.pone.0050717

Menalled LB, Kudwa AE, Miller S, Fitzpatrick J, Watson-Johnson J, et al. (2012) Comprehensive Behavioral and Molecular Characterization of a New Knock-In Mouse Model of Huntington’s Disease: zQ175. PLoS ONE 7(12): e49838. doi:10.1371/journal.pone.0049838

Take a Look at the Latest Research Using MBF Systems (12/24-1/4)


Our systems were mentioned in 13 published research papers over the past couple of weeks.  Take a closer look at some of the research…

Stereo Investigator:

  • Acosta, S. A., Tajiri, N., Shinozuka, K., Ishikawa, H., Grimmig, B., Diamond, D., … & Borlongan, C. V. (2013). Long-Term Upregulation of Inflammation and Suppression of Cell Proliferation in the Brain of Adult Rats Exposed to Traumatic Brain Injury Using the Controlled Cortical Impact Model. PLOS ONE,8(1), e53376. doi:10.1371/journal.pone.0053376
  • Chtarto, A., Bockstael, O., Gebara, E., Vermoesen, K., Melas, C., Pythoud, C., … & Tenenbaum, L. (2013). An Adeno-Associated Virus-Based Intracellular Sensor of Pathological Nuclear Factor-κB Activation for Disease-Inducible Gene Transfer. PLOS ONE8(1), e53156. doi:10.1371/journal.pone.0053156
  • Hua, K., Schindler, M. K., McQuail, J. A., Forbes, M. E., & Riddle, D. R. (2012). Regionally Distinct Responses of Microglia and Glial Progenitor Cells to Whole Brain Irradiation in Adult and Aging Rats. PLOS ONE7(12), e52728. doi:10.1371/journal.pone.0052728
  • Khan, M. M., Dhandapani, K. M., Zhang, Q. G., & Brann, D. W. (2012). Estrogen regulation of spine density and excitatory synapses in rat prefrontal and somatosensory cerebral cortex [Abstract]. Steroids. doi: 10.1016/j.steroids.2012.12.005
  • Kumar, A., Stoica, B. A., Sabirzhanov, B., Burns, M. P., Faden, A. I., & Loane, D. J. (2012). Traumatic brain injury in aged animals increases lesion size and chronically alters microglial/macrophage classical and alternative activation states [Abstract]. Neurobiology of Aging. doi:10.1016/j.neurobiolaging.2012.11.013
  • Lee, N. C., Shieh, Y. D., Chien, Y. H., Tzen, K. Y., Yu, I., Chen, P. W., … & Hwu, W. L. (2012). Regulation of the dopaminergic system in a murine model of aromatic L-amino acid decarboxylase deficiency [Abstract]. Neurobiology of Disease. doi:10.1016/j.nbd.2012.12.005
  • Littrell, O. M., Granholm, A. C., Gerhardt, G. A., & Boger, H. A. (2013). Glial cell-line derived neurotrophic factor (GDNF) replacement attenuates motor impairments and nigrostriatal dopamine deficits in 12-month-old mice with a partial deletion of GDNFPharmacology Biochemistry and Behavior. doi:10.1016/j.pbb.2012.12.022
  • Uzkeser, M., Sahin, H., Ozogul, B., Cayir, Y., Alper, F., & Emet, M. (2013). Defining the percentage of intra-abdominal hemorrhage in abdominal computerized tomography using stereology in patients with blunt liver injury and determining its relationship with outcomes [Abstract]. The Journal of Trauma and Acute Care Surgery74(1), 224-229. doi: 10.1097/TA.0b013e318270df0e


  • Fan, C., Zheng, Y., Cheng, X., Qi, X., Bu, P., Luo, X., … & Cao, Q. (2013). Transplantation of D15A-Expressing Glial-Restricted-Precursor-Derived Astrocytes Improves Anatomical and Locomotor Recovery after Spinal Cord Injury. International Journal of Biological Sciences9(1), 78. doi:10.7150/ijbs.5626
  • Kawabe, T., Kawabe, K., & Sapru, H. N. (2012). Effect of Barodenervation on Cardiovascular Responses Elicited from the Hypothalamic Arcuate Nucleus of the Rat. PLOS ONE7(12), e53111. doi:10.1371/journal.pone.0045180
  • Lopes, A., Torres, D. B., Rodrigues, A. J., Cerqueira, J. J., Pêgo, J. M., Sousa, N., … & Boer, P. A. (2012). Gestational protein restriction induces ca3 dendritic atrophy in dorsal hippocampal neurons but does not alter learning and memory performance in adult offspring. International Journal of Developmental Neuroscience. doi: 10.1016/j.ijdevneu.2012.12.003
  • Maseko, B. C., Jacobs, B., Spocter, M. A., Sherwood, C. C., Hof, P. R., & Manger, P. R. (2012). Qualitative and Quantitative Aspects of the Microanatomy of the African Elephant Cerebellar Cortex [Abstract]. Brain, Behavior and Evolution. doi: 10.1159/000345565
  • Miao, S., Chen, R., Ye, J., Tan, G. H., Li, S., Zhang, J., … & Xiong, Z. Q. (2013). The Angelman Syndrome Protein Ube3a Is Required for Polarized Dendrite Morphogenesis in Pyramidal Neurons. The Journal of Neuroscience,33(1), 327-333. doi:10.1523/jneurosci.2509-12.2013

Take a look at the latest research using MBF systems (Week of 12/10)

bookshelves-at-the-library_w482_h725Our systems were mentioned in 10 published research papers last week.  Take a closer look at some of the research…


Stereo Investigator:



Take a look at the latest research using MBF systems (Week of 12/3)

bookshelves-at-the-library_w482_h725Our systems were mentioned in 13 published research papers last week.  Take a closer look at some of the research.



Stereo Investigator:


 CX 9000 video camera system:




Unveiling WormLab 2.0, the Latest Version of our Groundbreaking Worm Tracking Software

We are delighted to introduce WormLab 2.0.  Our latest version of WormLab features an intuitive step-by-step workflow to analyze the locomotion and behavior of C. elegans and is now available to Mac and Windows 8 users. We’ve worked hard to improve our tracking algorithms, particularly our self-overlap and entanglement algorithms.  We’ve added whole plate mode for tracking many worms on a plate and added extensive camera support for over 1,400 different camera models.  Tracking is much faster throughout and the 64-bit version is even faster than the 32-bit version.

“Researchers are often overwhelmed by the painstaking frame by frame data collection process, often performed over hundreds of frames, which can be a tedious process.  With WormLab, video capture and tracking only take minutes so that researchers can focus on the meaningful part – analysis and interpretation.  Furthermore, the collected data is easily exported for more custom analysis to other software like Excel or Matlab,” says Jeff Sprenger, VP of Research.

Learn more about WormLab from our website and try WormLab 2.0 for free  for 30 days with full technical support.


MBF Bioscience Featured on Vermont Public Television

MBF Bioscience was featured in an episode of Emerging Science,  a television series that features Vermont scientists who expand human knowledge and help solve problems around the world.   The episode explored the link between traumatic brain injury and post traumatic stress disorder in military personnel coming back from war.

MBF Bioscience President Jack Glaser and Staff Scientist Susan Hendricks give us some perspective from a research standpoint as they briefly address the ways in which learning more about the injured brain could help find a cure. How does a brain injury affect the neurons in the affected area? Are there fewer neurons than there were before?  Has the shape of the neurons changed? Are there fewer connections between neurons than there were before injury? There is still so much more to explore.

Watch the episode online.