Upcoming Webinar: Stereology Concepts & Probe Selection

Area and Volume Estimation in Stereo InvestigatorStereology is considered the gold standard methodology for unbiased and precise quantification of microscopic biological features of tissues. Yet, researchers are often confused about how to properly design an experiment which effectively and efficiently employs stereology.  This webinar is designed to demystify the core concepts of stereology ( isotropy, systematic random sampling, tissue preparation, bias) to help participants confidently design their next experiment.

In this webinar led by Drs. Susan Tappan and Dan Peruzzi, participants will learn:

  • how to choose the correct probes for estimation of object number, length, surface  area, and regional volume.
  • how to select the most suitable type of tissue sectioning.
  • how to perform systematic sampling properly.

Go to our website to register for our free webinar “Stereology Concepts & Probe Selection” which will take place Thursday, March 28 at 12 noon US Eastern Standard Time.

You may also be interested in watching our previous webinars.

Take a Look at the Latest Research Using MBF Systems (week of 3/2/13)

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Our systems were mentioned in 19 published research papers last week alone.  Take a closer look at some of the research…

 Stereo Investigator:

  • Alexander, M., Smith, A. L., Rosenkrantz, T. S., & Fitch, R. H. (2013). Therapeutic Effect of Caffeine Treatment Immediately Following Neonatal Hypoxic-Ischemic Injury on Spatial Memory in Male Rats. Brain Sciences3(1), 177-190. doi: 10.3390/brainsci3010177
  • Bauernfeind, A. L., de Sousa, A. A., Avasthi, T., Dobson, S. D., Raghanti, M. A., Lewandowski, A. H., … & Sherwood, C. C. (2013). A volumetric comparison of the insular cortex and its subregions in primates. Journal of Human Evolution. doi: 10.1016/j.jhevol.2012.12.003
  • Contet, C., Kim, A., Le, D., Iyengar, S. K., Kotzebue, R. W., Yuan, C. J., … & Mandyam, C. D. (2013). μ‐Opioid receptors mediate the effects of chronic ethanol binge drinking on the hippocampal neurogenic niche. Addiction Biology. doi: 10.1111/adb.12040                                               Continue reading “Take a Look at the Latest Research Using MBF Systems (week of 3/2/13)” »

Celebrating 50 Years of the Computer Microscope (1963-2013) – Part I: The First Semi-Automatic System for Neuroanatomical Analysis

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Original figures published with permission from Dr. Ed Glaser

In 1963, Dr. Ed Glaser (co-founder of MBF Bioscience) and Dr. Hendrik van der Loos were at the John Hopkins Medical School putting the final touches on the first computer microscope, an analog computer connected to a light microscope. It was described as a system for attaching X-Y-Z transducers to a microscope stage, tracing the branches of a Golgi-stained neuron and outputting the result to a plotter (learn more about the origins of Neurolucida). The microscope stage was equipped with high-linearity linear motion transducers yielding output voltages proportional to the position of the microscope stage in all three dimensions. The computation of distances was performed by using a chord approximation to the curvilinear dendrites whose lengths were to be determined. Continue reading “Celebrating 50 Years of the Computer Microscope (1963-2013) — Part I: The First Semi-Automatic System for Neuroanatomical Analysis” »

Take a Look at the Latest Research Using MBF Systems (week of 2/25/13)

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Our systems were mentioned in 14 published research papers last week alone.  Take a closer look at some of the research…

Neurolucida:

MBF System for Analyzing Whole Sections of Human Brain Tissue Installed for a Neuropsychiatry Research Program in Texas

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Samantha Sachsenmayer, Research Assistant & Dr. Jeff Browning, Scientist, were trained by Dr. Dan Peruzzi during the installation.

An MBF Bioscience system for analyzing human brain tissue was recently installed at Texas A&M University System Health Sciences Center College of Medicine (TAMUSCOM). Dr. Dan Peruzzi, Staff Scientist, integrated Stereo Investigator with a Zeiss Vario microscope, camera, and a custom-made Ludl motorized stage that can hold up to 8″x 8″ slides – large enough for whole human brain sections – for Dr. Keith Young’s lab. In addition to the installation, Dr. Peruzzi taught the lab members how to use the system and demonstrated how to acquire SRS image stacks which are image stacks suitable for stereology.

Dr. Young’s lab is a key component of the Neuropsychiatry Research Program supported by TAMUSCOM and the Central Texas Veterans Health Care System. The  Neuropsychiatry Research Program focuses on translational and clinical research with an emphasis on schizophrenia and other brain diseases.  It has core facilities for stereological analysis of neuropathological tissue, immunohistochemistry, neurochemistry, behavioral pharmacology, and clinical psychopharmacology trials.

We’re pleased to be able to provide a specialized system for Dr. Young and his team.

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Take a Look at the Latest Research Using MBF Systems (week of 2/18/13)

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Our systems were mentioned in 10 published research papers last week alone.  Take a closer look at some of the research…

Neurolucida:

  • Garske, A. K., Lawyer, C. R., Peterson, B. M., & Illig, K. R. (2013). Adolescent Changes in Dopamine D1 Receptor Expression in Orbitofrontal Cortex and Piriform Cortex Accompany an Associative Learning Deficit. PLoS ONE8(2). doi: 10.1371/journal.pone.0056191
  • Négyessy, L., Pálfi, E., Ashaber, M., Palmer, C., Jákli, B., Friedman, R. M., … & Roe, A. W. (2013). Intrinsic horizontal connections process global tactile features in the primary somatosensory cortex: Neuroanatomical evidence.Journal of Comparative Neurology. doi: 10.1002/cne.23317

Continue reading “Take a Look at the Latest Research Using MBF Systems (week of 2/18/13)” »

Take a Look at the Latest Research Using MBF Systems (week of 2/11/13)

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Our systems were mentioned in 21 published research papers last week alone.  Take a closer look at some of the research…

Neurolucida:

  • Abbott, S. B., DePuy, S. D., Nguyen, T., Coates, M. B., Stornetta, R. L., & Guyenet, P. G. (2013). Selective Optogenetic Activation of Rostral Ventrolateral Medullary Catecholaminergic Neurons Produces Cardiorespiratory Stimulation in Conscious Mice. The Journal of Neuroscience33(7), 3164-3177. doi: 10.1523/​JNEUROSCI.1046-12.2013
  • Barr, T. P., Albrecht, P. J., Hou, Q., Mongin, A. A., Strichartz, G. R., & Rice, F. L. (2013). Air-Stimulated ATP Release from Keratinocytes Occurs through Connexin Hemichannels. PLOS ONE8(2), e56744. doi: 10.1371/journal.pone.0056744
  • De Lorme, K. C., & Sisk, C. L. (2013). Pubertal testosterone programs context-appropriate agonistic behavior and associated neural activation patterns in male Syrian hamsters. Physiology & Behavior. doi: 10.1016/j.physbeh.2013.02.003
  • Favero, M., & Castro-Alamancos, M. A. (2013). Synaptic Cooperativity Regulates Persistent Network Activity in Neocortex. The Journal of Neuroscience33(7), 3151-3163. doi: 10.1523/​JNEUROSCI.4424-12.2013

Continue reading “Take a Look at the Latest Research Using MBF Systems (week of 2/11/13)” »

Take a Look at the Latest Research Using MBF Systems (week of 2/4/13)

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Our systems were mentioned in 13 published research papers last week alone.  Take a closer look at some of the research…

 

 

Neurolucida:

  • Brumberg, C. (2012). Chia-Chien Chen, Danny Tam & Joshua. Brain Struct Funct217, 435-446. doi: 10.1007/s00429-011-0342-9
  • DeFelipe, J., López-Cruz, P. L., Benavides-Piccione, R., Bielza, C., Larrañaga, P., Anderson, S., … & Ascoli, G. A. (2013). New insights into the classification and nomenclature of cortical GABAergic interneurons. Nature Reviews Neuroscience. doi: doi:10.1038/nrn3444
  • Ibi, D., Nagai, T., Nakajima, A., Mizoguchi, H., Kawase, T., Tsuboi, D., … & Yamada, K. (2013). Astroglial IFITM3 mediates neuronal impairments following neonatal immune challenge in mice. Glia. doi: 10.1002/glia.22461
  • Nakamoto, K. T., Mellott, J. G., Killius, J., Storey-Workley, M. E., Sowick, C. S., & Schofield, B. R. (2013). Analysis of excitatory synapses in the guinea pig inferior colliculus: a study using electron microscopy and GABA immunocytochemistry. Neuroscience. doi: 10.1016/j.neuroscience.2013.01.061
  • Szucs, P., Luz, L. L., Pinho, R., Aguiar, P., Antal, Z., Tiong, S. Y., … & Safronov, B. V. (2013). Axon diversity of lamina I local‐circuit neurons in the lumbar spinal cord. Journal of Comparative Neurology. doi: 10.1002/cne.23311
  • Villar-Cerviño, V., Molano-Mazón, M., Catchpole, T., Valdeolmillos, M., Henkemeyer, M., Martínez, L. M., … & Marín, O. (2013). Contact Repulsion Controls the Dispersion and Final Distribution of Cajal-Retzius Cells. Neuron,77(3), 457-471. doi: 10.1016/j.neuron.2012.11.023

 

Stereo Investigator:

  • Brenhouse, H. C., Lukkes, J. L., & Andersen, S. L. (2013). Early Life Adversity Alters the Developmental Profiles of Addiction-Related Prefrontal Cortex Circuitry. Brain Sciences3(1), 143-158. doi: 10.3390/brainsci3010143
  • Dursun, İ., Jakubowska-Doğru, E., Elibol-Can, B., van der List, D., Chapman, B., Qi, L., & Berman, R. F. (2013). Effects of early postnatal alcohol exposure on the developing retinogeniculate projections in C57BL/6 mice. Alcohol. doi: 10.1016/j.alcohol.2012.12.013
  • Jonsson, S., Wiberg, R., McGrath, A. M., Novikov, L. N., Wiberg, M., Novikova, L. N., & Kingham, P. J. (2013). Effect of Delayed Peripheral Nerve Repair on Nerve Regeneration, Schwann Cell Function and Target Muscle Recovery.PLOS ONE8(2), e56484. doi:10.1371/journal.pone.0056484
  • Raman, L., Kong, X., & Kernie, S. G. (2013). Pharmacological inhibition of the mTOR pathway impairs hippocampal development in mice. Neuroscience Letters. doi: 10.1016/j.neulet.2013.01.045
  • Roth, T. C., Chevalier, D. M., LaDage, L. D., & Pravosudov, V. V. (2013). Variation in hippocampal glial cell numbers in food‐caching birds from different climates. Developmental Neurobiology. doi: 10.1002/dneu.22074
  • Wang, S., Bates, J., Li, X., Schanz, S., Chandler-Militello, D., Levine, C., … & Goldman, S. A. (2013). Human iPSC-Derived Oligodendrocyte Progenitor Cells Can Myelinate and Rescue a Mouse Model of Congenital Hypomyelination. Cell Stem Cell12(2), 252-264. doi: 10.1016/j.stem.2012.12.002

 

Neuroinformatica (now replaced by Biolucida Cloud):

  • Bonser, S. P., de Permentier, P., Green, J., Velan, G. M., Adam, P., & Kumar, R. K. (2013). Engaging students by emphasising botanical concepts over techniques: innovative practical exercises using virtual microscopy. Journal of Biological Education, (ahead-of-print), 1-5. doi: 10.1080/00219266.2013.764344

Take a Look at the Latest Research Using MBF Systems (week of 1/28/13)

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Our systems were mentioned in 13 published research papers last week alone.  Take a closer look at some of the research…

 

 

Neurolucida:

  • Akyüz, N., Rost, S., Mehanna, A., Bian, S., Loers, G., Oezen, I., … & Schachner, M. (2013). Dermatan 4- O-sulfotransferase1 ablation accelerates peripheral nerve regeneration. Experimental Neurology. doi: 10.1016/j.expneurol.2013.01.025
  • Carmel, J. B., Kimura, H., Berrol, L. J., & Martin, J. H. (2013). Motor cortex electrical stimulation promotes axon outgrowth to brain stem and spinal targets that control the forelimb impaired by unilateral corticospinal injury. European Journal of Neuroscience. doi: 10.1111/ejn.12119
  • Cisbani, G., Freeman, T. B., Soulet, D., Saint-Pierre, M., Gagnon, D., Parent, M., … & Cicchetti, F. (2013). Striatal allografts in patients with Huntington’s disease: impact of diminished astrocytes and vascularization on graft viability.Brain. doi: 10.1093/brain/aws359
  • Cullen, C. L., Burne, T. H., Lavidis, N. A., & Moritz, K. M. (2013). Low Dose Prenatal Ethanol Exposure Induces Anxiety-Like Behaviour and Alters Dendritic Morphology in the Basolateral Amygdala of Rat Offspring. PLOS ONE8(1), e54924. doi:10.1371/journal.pone.0054924
  • Hughes, D. I., Boyle, K. A., Kinnon, C. M., Bilsland, C., Quayle, J. A., Callister, R. J., & Graham, B. A. (2013). HCN4 subunit expression in fast-spiking interneurons of the rat spinal cord and hippocampus. Neuroscience. doi: 10.1016/j.neuroscience.2013.01.028

Stereo Investigator:

  • Carmel, J. B., Kimura, H., Berrol, L. J., & Martin, J. H. (2013). Motor cortex electrical stimulation promotes axon outgrowth to brain stem and spinal targets that control the forelimb impaired by unilateral corticospinal injury. European Journal of Neurosciencedoi: 10.1111/ejn.12119
  • Chen, C. D., Sloane, J. A., Li, H., Aytan, N., Giannaris, E. L., Zeldich, E., … & Abraham, C. R. (2013). The Antiaging Protein Klotho Enhances Oligodendrocyte Maturation and Myelination of the CNS. The Journal of Neuroscience33(5), 1927-1939. doi: 10.1523/​JNEUROSCI.2080-12.2013
  • Gould, K. L., Gilbertson, K. E., Hrvol, A. J., Nelson, J. C., Seyfer, A. L., Brantner, R. M., & Kamil, A. C. (2013). Differences in Relative Hippocampus Volume and Number of Hippocampus Neurons among Five Corvid SpeciesBrain, Behavior and Evolution81(1), 56-70.
  • Gyengesi, E., Andrews, Z. B., Paxinos, G., & Zaborszky, L. (2013). Distribution of secretagogin-containing neurons in the basal forebrain of mice, with special reference to the cholinergic corticopetal system. Brain Research Bulletin. doi: 10.1016/j.brainresbull.2013.01.009
  • He, Z., Ferguson, S. A., Cui, L., Greenfield Jr, L. J., & Paule, M. G. (2013). Role of Neural Stem Cell Activity in Postweaning Development of the Sexually Dimorphic Nucleus of the Preoptic Area in Rats. PLOS ONE8(1), e54927. doi:10.1371/journal.pone.0054927
  • Inan, M., Blázquez-Llorca, L., Merchán-Pérez, A., Anderson, S. A., DeFelipe, J., & Yuste, R. (2013). Dense and Overlapping Innervation of Pyramidal Neurons by Chandelier Cells. The Journal of Neuroscience33(5), 1907-1914. doi: 10.1523/​JNEUROSCI.4049-12.2013
  • Karasinska, J. M., de Haan, W., Franciosi, S., Ruddle, P., Fan, J., Kruit, J. K., … & Hayden, M. R. (2013). ABCA1 influences neuroinflammation and neuronal death. Neurobiology of Disease. doi: 10.1016/j.nbd.2013.01.018
  • Morales-Medina, J. C., Juarez, I., Venancio-García, E., Cabrera, S. N., Menard, C., Yu, W., … & Quirion, R. (2013). Impaired structural hippocampal plasticity is associated with emotional and memory deficits in the olfactory bulbectomized rat. Neuroscience. doi: 10.1016/j.neuroscience.2013.01.037
  • Wang, X., Zielinski, M. C., Misawa, R., Wen, P., Wang, T. Y., Wang, C. Z., … & Hara, M. (2013). Quantitative Analysis of Pancreatic Polypeptide Cell Distribution in the Human Pancreas. PLOS ONE8(1), e55501. doi:10.1371/journal.pone.0055501

 

MBF Bioscience Supports the 4th Vermont Brain Bee

brain-bee-2011-jn-photo-3Thirty-six high school students passionate about neuroscience will be competing at the 4th annual Vermont Brain Bee on February 9th, 2013 at the University of Vermont College of Medicine. Participants will be asked questions about a wide variety of topics: anatomy and development, learning and memory, stress, types of research, neurogenerative disorders, etc. They will also get the opportunity to engage in neuroscience activities and to meet with neuroscience faculty and graduate students. The winner will then compete in the National Brain Bee, an event that takes place in March during Brain Awareness Week.

Participation in the Vermont Brain Bee is on the rise thanks to the burgeoning of neuroscience clubs in Vermont high schools thanks to a grant from the Vermont Community Foundation. Brain injury survivor Lisa Bernardin has been instrumental in promoting the creation of these clubs;  she’s been giving talks about her experience and involved neuroscience undergraduate students in her presentations in an effort to better engage the high school audience.

We are proud to renew our support for this event since we, along with the Brain Bee’s organizers, believe that inspiring young people to engage in careers contributing to neuroscience is imperative.

The Vermont Brain Bee is coordinated by Bernardin with the support of UVM faculty and the Vermont Chapter of the Society for Neuroscience .

Find more information about the 2013 event here and watch WCAX .