Stereology Workshop at Obafemi Awolowo University in Nigeria

CaptureStereology Symposium in Nigeria

Dr. Jose Maldonado, Head of MBF Bioscience Latin America and Africa, and Dr. Abraham A.A. Osinubi, Associate Professor, University of Lagos will be hosting a stereology workshop at Obafemi Awolowo University in Nigeria on Sept 16 – 18. The workshop will cover the basics of stereology and how to practically apply it to scientific research.

This is a unique opportunity for attendees to learn from two expert stereologists. Anyone interested in stereology is welcome to attend, including undergraduate and post-graduate students and faculty/researchers from biological and material science, geologists, pathologists, and statisticians.

To register for this workshop, send an email to stating your full name, institution, email address, phone number and status (Student/Faculty).

More information can be found here in the workshop brochure.

First System for Imaging and Analyzing Large Mammalian Brain Tissue Installed in South Africa

Dr. Paul Manger's new system developed by MBF Bioscience for analyzing large mammalian brain specimens

Dr. Paul Manger’s new system developed by MBF Bioscience for analyzing large mammalian brain specimens

Dr. Paul Manger, Research Professor at the University of the Witwatersrand, South Africa, studies the brain anatomy of various animals to better understand the relationship between brain structure and function. Some of the mammals he studies have very large brains that don’t fit under a conventional research microscope. A team of scientists and microscopy experts at MBF Bioscience developed a new microscope system that enables Dr. Manger to analyze and image whole sections of large brain tissue. This system is the first for imaging and analyzing entire tissue sections of large mammalian brains. It will give Dr. Manger unprecedented analyses of African mammal brains and a way to share large images of tissue specimens with collaborators or a global audience. Continue reading “First System for Imaging and Analyzing Large Mammalian Brain Tissue Installed in South Africa” »

Satellite Symposium at SfN: Enhancing the Reproducibility of Your Research Results with Stereology


Recent headlines decry the alarming amount of irreproducible data in published research papers. MBF Bioscience is hosting a symposium addressing the topic on Sunday, October 18 at 6:30pm entitled “Quantitative Microscopy: Enhancing the Reproducibility of Your Research Results with Stereology.” This symposium, which is a satellite event at the annual Society for Neuroscience meeting, will address how researchers can use stereology to obtain accurate, reproducible data about their histological tissue specimens to drive meaningful discovery by the scientific community at large.

It will be an evening full of engaging speakers with significant expertise in various aspects of stereology, including: stereology theory, the application of stereology in neuroscience research, and why some funding agencies require stereology. Attendees will have a chance to interact with the speakers during the question and answer session following the presentations.

Stereology is the gold standard method for quantifying the number of cells, length of fibers, and the area and volume of structures or regions in tissue specimens. Attend this symposium to learn how it can help you obtain accurate, reproducible data for your research study.

Webinar Video: Automated 3D Neuron Reconstruction


Confocal image of a mouse cerebellum optically cleared with CLARITY. Neurons were reconstructed with Neurolucida 360.


In this webinar, Dr. Dara Dickstein, Assistant Professor at Icahn School of Medicine at Mount Sinai, and Dr. Susan Tappan, Staff Scientist and Neurolucida 360 Product Manager discuss imaging protocols and techniques for automated 3D neuron reconstruction.

Watch the webinar on YouTube

The goal is to help you develop an imaging and quantification strategy for your own research to increase the throughput of your morphologic study.

Watch this webinar to learn:

+ Different methods for tissue preparation
+ Imaging protocols optimized for automated neuron reconstruction
+ How to use Neurolucida 360 – our new automated software for achieving accurate 3D reconstructions of somas, dendrites, spines, and axons


Interactive Probe Selector for Stereology

probe selectorSelecting a probe for stereology is an important decision that should be made before you section your tissue. Some probes require thick sections and others require thin sections. We created an Interactive Probe Selector to help you choose the appropriate probe for your research. Give it a try and let us know what you think.Area and Volume Estimation in Stereo Investigator

Leica Microsystems and MBF Bioscience announce a partnership in the United States to offer analysis systems for life science research

Leica and MBF partner to offer life science research solutionsLeica Microsystems’ microscopes can now fully integrate with Neurolucida and Stereo Investigator software for analyzing cells in neuroscience research.

Buffalo Grove, IL, USA.    Leica Microsystems and MBF Bioscience today announce a partnership to offer Leica Microsystems’ microscopes fully integrated with MBF’s Neurolucida and Stereo Investigator software in the United States.  This partnership gives researchers new tools to analyze tissue specimens and discover information about the brain, spinal cord, eye, and lung.

“Combining our analysis software with Leica microscopes means that researchers can get a fully integrated system for collecting accurate, reliable data from their tissue specimens,” says Jack Glaser, President of MBF Bioscience. “We are pleased to partner with Leica Microsystems to provide integrated systems. As leading companies in the fields of stereology and neuron tracing, we are proud to offer systems with such a distinguished company as Leica Microsystems.”

This tight integration allows Neurolucida and Stereo Investigator software to display the microscope image onto a computer monitor for analysis. The software automatically adjusts Leica microscope components, such as the fluorescent filters and objective lenses, based on the research task. Integration is necessary for researchers performing unbiased stereology with Stereo Investigator, which requires the software to drive the motorized stage to random locations on the tissue specimen for unbiased quantification of cell populations. Researchers using Neurolucida software for neuron reconstruction, a technique to analyze the shape and size of neurons, will save a lot of time using a fully integrated system. For example, Neurolucida will automatically move the microscope stage when a researcher reconstructs a neuron that extends beyond the field of view.

Continue reading “Leica Microsystems and MBF Bioscience announce a partnership in the United States to offer analysis systems for life science research” »

Popular open access virtual slide collection has a new home

We are happy to announce that Biolucida, our learning management software, is now hosting the Iowa Virtual Slidebox. This vast repository of digitized tissue specimens is open to the public and is free of charge. Virtual slides in this collection are used by educators around the world to teach histopathology and histology.


“Educators and students will now have easier and quicker access to virtual slides from the Iowa Virtual Slidebox,” said Dr. Nathan O’Connor, Biolucida product manager. “In addition, MBF can offer fully annotated versions of these slides that highlight specific structures or areas of interest within the specimens.”

The collection was curated by Fred Dee, MD, a pathologist at the University of Iowa who is a pioneer in using virtual slides for medical education. “We created this virtual slide collection with a grant from the National Library of Medicine to provide an efficient open source alternative for histology and histopathology course directors around the world, as traditional laboratory based glass slide microscopy was beginning to disappear from curricula,” said Dr. Dee. “The Iowa Virtual Slidebox has been hosted by MBF Bioscience since 2001. We are extremely pleased that MBF Bioscience will continue that tradition with their new Biolucida software.”

Biolucida is a learning platform that uses virtual slides  ̶  high-resolution digital images of tissue specimens  ̶  to simulate the experience of using a light microscope, complete with the ability to focus in z through specimens. It supports images that are hundreds of gigabytes in size, and it is web-based so educators and students can view the slides from any computer with an internet connection.

To download the app and view the Iowa Collection on Biolucida at full resolution, free of charge, please fill out this short form.

First Name (required)

Last Name (required)

Email (required)

Neurons in the Basal Forebrain are Exquisitely Organized

File:Nucleus basalis of Meynert - intermed mag.jpg

A micrograph of the nucleus basalis of Meynert, a group of neurons in the basal forebrain that produces most of the acetylcholine supplied to the cerebral cortex.
Image from Wikimedia Commons

The loss of cholinergic neurons is one of the earliest pathological events of Alzheimer’s disease. Cholinergic neurons in the basal forebrain supply the cerebral cortex with acetylcholine, an important neurotransmitter that plays a role in learning, memory, and attention. Details about the function and organization of basal forebrain (BF) neurons are not well understood, but Dr. Laszlo Zaborszky has recently uncovered new information about the structure of this complex area of the brain.

In a paper published in Cerebral Cortex, Dr. Zaborszky and his team report that they discovered exquisite organization in the basal forebrain of rats; the extent of overlap between basal forebrain neuronal populations correlates with the connectivity strength between their cortical targets. This means that basal forebrain neurons that overlap extensively project to frontal and posterior cortical areas that are strongly connected. Connectivity strength between cortical areas is defined by the number of neurons in a defined posterior cortical area that project to a defined area in the frontal cortex. Continue reading “Neurons in the Basal Forebrain are Exquisitely Organized” »

Microsoft ends support for Windows XP: What it means to our customers


Microsoft announced that it will discontinue support for Windows XP on April 8th, 2014. After this date, there will be no new security updates and your lab may be exposed to potential security risks and viruses.

If you have an MBF system that runs on Windows XP, please be aware that older cameras and stages might not be compatible with newer Windows operating systems. Please contact us at or 802-288-9290 before you upgrade to a more recent version of Windows.

For more information about the discontinuation of support for XP, please visit Microsoft’s website.