Researchers at Stanford use confocal stereology to study neurodevelopment

A Stereo Investigator system for confocal stereology was installed in Dr. Michelle Monje’s lab in the Department of Neurology and Neurological Sciences at Stanford University School of Medicine. Dr. Monje and her lab members will use the system to investigate the molecular and cellular mechanisms of postnatal neurodevelopment.

Dr. Julie Korich, staff scientist at MBF, installed Stereo Investigator on a Zeiss laser scanning confocal microscope and trained the lab members on how to use the system.

From top left: Chris Mount, Grant Lin, Ingrid Inpma, David Purger From bottom left: Elizabeth Qin, Viola Caretti, Lauren Wood

From top left: Chris Mount, Grant Lin, Ingrid Inpma, David Purger
From bottom left: Elizabeth Qin, Viola Caretti, Lauren Wood

During the training, Dr. Korich discussed how Stereo Investigator integrates with Zeiss’ microscope software, explained the Cavalieri probe for estimating regional volume, and showed the lab how to use Stereo Investigator to collect confocal image stacks in the systematic and random way that’s necessary for unbiased stereology. She also explained how to count cells from those image stacks and from 3D virtual slides with Stereo Investigator on a computer away from the microscope.

Click here to learn more about how researchers are using Stereo Investigator in their labs.

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